Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of IL-1α expression in non-transfected: (A) and human IL-1α transfected: (B) 293 whole cell lysates.Application
Antibodies tested include IL-1α (H-159): (A) and IL-1α (B-7): (B).| Product Name | IL-1α Antibody (H-159) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 113-271 representing mature IL-1α of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Two forms of interleukin-1, designated IL-1å and IL-1∫, have been described. Although encoded by distinct genes and exhibiting roughly only 25% sequence identity, IL-1å and IL-1∫ bind to the same receptor and seem to elicit similar biological responses. IL-1 production is generally thought to be associated with inflammation, but it has also been shown to be expressed during kidney development, thymocyte differentiation and cartilage degradation. IL-1 plays a critical role in the regulation of immune response and inflammation, acting as an activator of T and B lymphocytes and natural killer (NK) cells. In T cells, IL-1 stimulates the production of IL-2 and selectively inhibits IL-4 expression. IL-1 induces B cell proliferation and maturation, and immunoglobulin synthesis. NK cells require IL-1∫ for production of the anti- pathogen IFN-©. IL-1 has also been implicated in several pathological conditions including rheumatoid arthritis, inflammatory bowel disease and atherosclerosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of IL-1α of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 113-271 representing mature IL-1α of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 113-271 representing mature IL-1α of human origin |
| Entrez Gene | 3552 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of IL-1α expression in non-transfected: (A) and human IL-1α transfected: (B) 293 whole cell lysates.
Application
Antibodies tested include IL-1α (H-159): (A) and IL-1α (B-7): (B).| Application Notes | recommended for detection of IL-1α of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
