Application
Western blot analysis of Myt 1 expression in HeLa (A), K-562 (B) and SK-BR-3 (C) whole cell lysates.| Product Name | Myt 1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-304 mapping at the N-terminus of Myt 1 of human origin |
| Modification Notes | Phosphorylation of Cdc2 on threonine 14 and tyrosine 15 is required to maintain Cdc2 in an inactive state throughout the S and G2 phases of the cell cycle. The human Wee 1 protein, Wee 1 Hu, encodes a tyrosine-specific protein kinase that phosphorylates Cdc2 on tyrosine 15. Myt 1, a member of the Wee 1 family of protein kinases, has been shown to phosphorylate Cdc2 on both threonine 14 and tyrosine 15 in a cyclin-dependent manner. Activity of both Wee 1 Hu and Myt 1 is regulated during the cell cycle, suggesting that both proteins play a role in mitotic control. Dephosphorylation of Cdc2 on threonine 14 and tyrosine 15 in late G2 by Cdc25 then activates the Cdc2/cyclin B complex to allow entry into mitosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Myt 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-304 mapping at the N-terminus of Myt 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-304 mapping at the N-terminus of Myt 1 of human origin |
| Entrez Gene | 9088 |
|---|
Application
Western blot analysis of Myt 1 expression in HeLa (A), K-562 (B) and SK-BR-3 (C) whole cell lysates.| Application Notes | recommended for detection of Myt 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
