Application
Western blot analysis of Plk expression in non-transfected: (A) and human Plk transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Plk expression in non-transfected: (A) and human Plk transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Plk expression in non-transfected: (A) and mouse Plk transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Plk expression in K-562 (A) and A549 (B) whole cell lysates.| Product Name | Plk Antibody (H-152) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 261-412 mapping to an internal region of Plk of human origin |
| Modification Notes | Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Plk of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 261-412 mapping to an internal region of Plk of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 261-412 mapping to an internal region of Plk of human origin |
| Entrez Gene | 5347 |
|---|
Application
Western blot analysis of Plk expression in non-transfected: (A) and human Plk transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Plk expression in non-transfected: (A) and human Plk transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Plk expression in non-transfected: (A) and mouse Plk transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Plk expression in K-562 (A) and A549 (B) whole cell lysates.| Application Notes | recommended for detection of Plk of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
