| Product Name | Fat Antibody (D-295) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4826-5120 mapping near the C-terminus of Fat of Drosophila melanogaster origin |
| Modification Notes | Drosophila melanogaster, a proven and effective model for studying developmental and cellular processes common to higher eukaryotes, contains a genome encoding approximately 13,600 genes, which were elucidated from more than 120 megabases of euchromatin (1). These genes are organized among chromosomes 2, 3, 4, X, and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes, which are categorized based on the type of protein for which they encode, represent six major classifications, including intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated), and other functional proteins (2). Fat, an atypical cadherin, contains a putative signal sequence, 34 tandem cadherin domains, four EGF-like repeats, a transmembrane domain, and a novel cytoplasmic domain (3,4). Fat controls both cell growth and planar polarity, including mediating the proper orientation of wing hairs and ommatidia in the eye (3-7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Fat of Drosophila origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4826-5120 mapping near the C-terminus of Fat of Drosophila melanogaster origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4826-5120 mapping near the C-terminus of Fat of Drosophila melanogaster origin |
| Application Notes | recommended for detection of Fat of Drosophila origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
