Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of RARα expression in non-transfected: (A) and mouse RARα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RARα expression in SK-BR-3 (A) and MCF7 (B) nuclear extracts and AML-193 whole cell lysate (C).Application
Western blot analysis of RARα expression in non-transfected control (A) and RARα siRNA transfected (B) HeLa cells. Blot probed with RARα (C-20): . β Tubulin (D-10): used as specificity and loading control.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.Application
Antibodies tested include RARα (C-20): (A) and RXRα (D-20): (B,C).| Product Name | RARα Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of RARα1 and RARα2 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Retinoids (RA) are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. RAs activate the retinoic acid receptor (RAR) and retinoid X receptor (RXR) nuclear transcription factor families. Most retinoid forms activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RAR family members, which include RARå, RAR∫ and RAR©, have a high affinity for all transretinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. The human RARå gene maps to chromosome 17 and is implicated in the chromosomal translocation associated with acute promyelocytic leukemia (APL-M3). Specifically, the RARå gene is fused with the promyelocytic leukemia (PML) gene, which encodes the fusion protein PML/RARå. The PML/RARå fusion protein inhibits PML-dependent apoptotic pathways and halts myeloid differentiation at the promyelocytic stage. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of RARα1 and RARα2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of RARα1 and RARα2 of human origin |
| Entrez Gene | 5914 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of RARα expression in non-transfected: (A) and mouse RARα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RARα expression in non-transfected: (A) and human RARα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RARα expression in SK-BR-3 (A) and MCF7 (B) nuclear extracts and AML-193 whole cell lysate (C).
Application
Western blot analysis of RARα expression in non-transfected control (A) and RARα siRNA transfected (B) HeLa cells. Blot probed with RARα (C-20): . β Tubulin (D-10): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing nuclear and cytoplasmic staining.
Application
Antibodies tested include RARα (C-20): (A) and RXRα (D-20): (B,C).| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
