Application
Western blot analysis of NHPX expression in Jurkat (A), Hep G2 (B), SW480 (C), HeLa (D) and MCF7 (E) nuclear extracts.Application
Western blot analysis of NHPX expression in Jurkat whole cell lysate.| Product Name | NHPX Antibody (N-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of NHPX of human origin |
| Modification Notes | NHPX, also known as NHP2L1 (NHP2 non-histone chromosome protein 2-like 1), FA-1, 15.5K, OTK27, SNU13, SPAG12 or SNRNP15-5, is a 128 amino acid protein belonging to the ribosomal protein L7Ae family. NHPX localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. Ubiquitously expressed, NHPX binds to the 5’-stem-loop of U4 snRNA and may be involved in the late stage of spliceosome assembly. Following RNA binding, NHPX undergoes a conformational change and is recruited to introns, where NHPX is required for the subsequent recruitment of PRPF31 and the activation of the spliceosome complex. NHPX is expressed as two isoforms produced by alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NHPX of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of NHPX of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of NHPX of human origin |
| Entrez Gene | 4809 |
|---|
Application
Western blot analysis of NHPX expression in Jurkat (A), Hep G2 (B), SW480 (C), HeLa (D) and MCF7 (E) nuclear extracts.
Application
Western blot analysis of NHPX expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of NHPX of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
