Application
Western blot analysis of SAPK4 expression in non-transfected 293: (A), human SAPK4 transfected 293: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of SAPK4 expression in non-transfected 293: (A), human SAPK4 transfected 293: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of SAPK4 expression in non-transfected: (A) and human SAPK4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SAPK4 expression in non-transfected: (A) and mouse SAPK4 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include SAPK4 (N-17): (A) and SAPK4 (H-60): (B).Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Product Name | SAPK4 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 306-365 of SAPK4 of human origin |
| Modification Notes | Lipopolysaccharide has been shown to induce tyrosine phosphorylation of a unique protein, which is designated p38. p38 is a member of the MAP kinase family with features most closely resembling those of the Saccharo-myces cerevisiae protein HOG1. p38 and HOG1 share a TGY phosphorylation sequence, whereas most other MAP kinase family proteins have a TEY sequence. A related protein, p38b, has been shown to phosphorylate ATF-2 at a 20-fold higher rate than p38, suggesting distinct substrate preferences. Stress activated protein kinase-4, or SAPK4, also designated p38d, is a related protein that is phosphorylated by MKK6 in response to cytokines and cellular stresses. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SAPK4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 306-365 of SAPK4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 306-365 of SAPK4 of human origin |
| Entrez Gene | 5603 |
|---|
Application
Western blot analysis of SAPK4 expression in non-transfected 293: (A), human SAPK4 transfected 293: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of SAPK4 expression in non-transfected 293: (A), human SAPK4 transfected 293: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of SAPK4 expression in non-transfected: (A) and human SAPK4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SAPK4 expression in non-transfected: (A) and mouse SAPK4 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include SAPK4 (N-17): (A) and SAPK4 (H-60): (B).
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.| Application Notes | recommended for detection of SAPK4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
