Application
Antibodies tested include p-Ac-Histone H3 (Ser 11/Lys 15): (A,B,C,D,E,F,G) and Histone H3 (FL-136): (H,I,J,K,L,M,N).Application
Western blot analysis of Histone Ac-H3.3A phosphorylation in non-transfected 293T: (A), human Histone H3.3A transfected 293T: (B) and NTERA-2 cl.D1 (C) whole cell lysates.Application
Western blot analysis of Histone H3.3B phosphorylation in non-transfected 293T: (A), mouse Histone H3.3B transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of Histone H3.3B phosphorylation in non-transfected 293T: (A), mouse Histone H3.3B transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Histone cluster 1 H3E phosphorylation in non-transfected 293T: (A), human Histone cluster 1 H3E transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of phosphorylated Ac-Histone H3 expression in 293 (A) and HeLa (B) whole cell lysates.| Product Name | p-Ac-Histone H3 Antibody (Ser 11/Lys 15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to dually phosphorylated Ser 11 and aceylated Lys 15 of Histone H3 of human origin |
| Modification | Phosphoralated |
| Modification Notes | In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histone H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at Lysine 14 and 18 and Histone H4 at Lysine 5 and 8. Histone H4 may also be acetylated at Lysine 12 and 16, and the involvement of acetylated H4 with Histone H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling. Phosphorylation of Histone H3 is involved in chromosome condensation during mitosis. Histone H3 phosphorylation correlates to the expression of immediate-early genes such as c-Jun, c-Fos and c-Myc. Research indicates that MSK1, a growth and stress activated kinase, also phosphorylates Histone H3 in vitro. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 11 phosphorylated and Lys 15 acetylated Histone H3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to dually phosphorylated Ser 11 and aceylated Lys 15 of Histone H3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to dually phosphorylated Ser 11 and aceylated Lys 15 of Histone H3 of human origin |
Application
Antibodies tested include p-Ac-Histone H3 (Ser 11/Lys 15): (A,B,C,D,E,F,G) and Histone H3 (FL-136): (H,I,J,K,L,M,N).
Application
Western blot analysis of Histone Ac-H3.3A phosphorylation in non-transfected 293T: (A), human Histone H3.3A transfected 293T: (B) and NTERA-2 cl.D1 (C) whole cell lysates.
Application
Western blot analysis of Histone H3.3B phosphorylation in non-transfected 293T: (A), mouse Histone H3.3B transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of Histone H3.3B phosphorylation in non-transfected 293T: (A), mouse Histone H3.3B transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Histone cluster 1 H3E phosphorylation in non-transfected 293T: (A), human Histone cluster 1 H3E transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of phosphorylated Ac-Histone H3 expression in 293 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Ser 11 phosphorylated and Lys 15 acetylated Histone H3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
