Application
Western blot analysis of OPN1MW/LW expression in HeLa nuclear extract.| Product Name | OPN1MW/LW Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 206-260 mapping within an internal region of the opsin protein encoded by OPN1MW of human origin |
| Modification Notes | G protein-coupled receptors (GPCRs), which are characterized by containing seven transmembrane å helices, elicit G protein-mediated signaling cascades in response to a variety of stimuli. The opsin subfamily, which represents approximately 90 percent of all GPCRs, is comprised of photoreceptors that are activated by light. It includes the red-, green- and blue-sensitive opsins and rhodopsin. The opsin subfamily consists of an apoprotein covalently linked to 11-cis-retinal, which undergoes isomerization upon the absorption of photons. This isomerization leads to a conformational change of the protein, which results in the activation of hundreds of G proteins. Color is perceived in humans by three pigments, which localize to retinal cone photoreceptor cells. They are the blue-, green- and red-sensitive opsins, which are encoded by OPN1SW, OPN1MW and OPN1LW, respectively. Mutations in the OPN1MW and OPN1LW encoded opsins lead to the X-linked disorders protanopia and deuteranopia, respectively. Mutations in the OPN1SW encoded opsin leads to tritanopia, an autosomal dominant disorder, which is characterized by decreased sensitivity to blue light. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of the opsin proteins encoded by OPN1MW and OPN1LW of human origin, and the opsin protein encoded by Opn1mw of mouse and rat origin of mouse by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 206-260 mapping within an internal region of the opsin protein encoded by OPN1MW of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 206-260 mapping within an internal region of the opsin protein encoded by OPN1MW of human origin |
| Entrez Gene | 2652 |
|---|
Application
Western blot analysis of OPN1MW/LW expression in HeLa nuclear extract.| Application Notes | recommended for detection of the opsin proteins encoded by OPN1MW and OPN1LW of human origin, and the opsin protein encoded by Opn1mw of mouse and rat origin of mouse by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
