Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TAP1 expression in Raji (A), GA-10 (B) and IB4 (C) whole cell lysates.Application
Western blot analysis of TAP1 expression in Raji (A) and GA-10 (B) whole cell lysates.| Product Name | TAP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3-300 mapping at the N-terminus of TAP1 of human origin |
| Modification Notes | The transporter associated with antigen processing (TAP) is a member of the ATP-binding cassette (ABC) family of transmembrane transporters and is an essential component of the major histocompatability complex (MHC) class I antigen-presenting pathway (1). TAP consists of two structurally related subunits, TAP1 and TAP2, that associate into stable dimers and together they form a translocation pore for peptides in the endoplasmic reticulum (ER) membranes (2,3). The functional TAP transporter facilitates the translocation of peptides from the cytosol into the ER lumen for presentation to MHC class I molecules (4). Structurally, TAP1 and TAP2 contain an N-terminal transmembrane (TM) region, which together forms the TM pore, and a cytoplasmic peptide-binding pocket (5,6). In addition, the TAP transporter also contains several C-terminal nucleotide-binding domains (NBD), which bind and hydrolyze ATP and in turn, induce structural changes at the membrane to allow the passage of substrates into the ER (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TAP1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3-300 mapping at the N-terminus of TAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3-300 mapping at the N-terminus of TAP1 of human origin |
| Entrez Gene | 6890 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic and membrane staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TAP1 expression in Raji (A), GA-10 (B) and IB4 (C) whole cell lysates.
Application
Western blot analysis of TAP1 expression in Raji (A) and GA-10 (B) whole cell lysates.| Application Notes | recommended for detection of TAP1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
