Application
Antibodies tested include p-Ret (Tyr 1062)-R: (A,B,C) and Ret (H-300): (D,E,F).Application
Western blot analysis of Ret expression in non-transfected: (A) and human Ret transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ret expression in TT whole cell lysate.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of Ret expression in a Ret transfected whole cell lysate.| Product Name | Ret Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-330 mapping near the N-terminus of Ret of human origin |
| Modification Notes | The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. By alternative splicing, two isoforms of the Ret proto-oncogene product are generated. The isoforms differ from each other by having either 9 or 51 carboxy terminal amino acids. The Ret gene products include two glycosylated proteins and, in tunicamycin treated cells, a non-glycosylated protein consistent with the predicted Ret molecular weight based on sequence analysis. Tumor-specific rearrangements of the Ret proto-oncogene have been identified in papillary thyroid carcinomas leading to the formation of different transforming fusion proteins sharing the tyrosine kinase domain of Ret. In contrast to the Ret proto-oncogene, the rearranged forms are constitutively phosphorylated on tyrosine and are translocated from the membrane to the cytoplasm. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ret of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-330 mapping near the N-terminus of Ret of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-330 mapping near the N-terminus of Ret of human origin |
| Entrez Gene | 5979 |
|---|
Application
Antibodies tested include p-Ret (Tyr 1062)-R: (A,B,C) and Ret (H-300): (D,E,F).
Application
Western blot analysis of Ret expression in non-transfected: (A) and human Ret transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ret expression in TT whole cell lysate.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of Ret expression in a Ret transfected whole cell lysate.| Application Notes | recommended for detection of Ret of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
