Application
Western blot analysis of FBLIM1 expression in WI 38 whole cell lysate.| Product Name | FBLIM1 Antibody (H-73) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 301-373 mapping at the C-terminus of FBLIM1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | FBLIM1 (filamin binding LIM protein 1), also known as CAL (CSX-associated LIM), Mig-2-interacting protein or Migfilin, is a cytoplasmic protein that belongs to the LIM superfamily. Three isoforms exist for FBLIM1 due to alternative splicing events, namely FBLP-1A, FBLP-1 and FBLP-1B. All three isoforms are expressed in pancreas, kidney, placenta, lung, platelets and heart, while FBLP-1 is also expressed in brain and skeletal muscle. Depending on the isoform, FBLIM1 contains a proline-rich domain and two or three C-terminal LIM zinc-binding domains. FBLIM1 specifically localizes to cell-matrix adhesion sites and, via its C-terminus, interacts with Mig-2 functioning as an important scaffold protein. Via its N-terminus, FBLIM1 interacts with Filamin 3 and provides an anchoring site for actin filaments, linking cell-matrix adhesions with the actin cytoskeleton. In addition, FBLIM1 is capable of translocating to the nucleus and regulating gene expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIPWB |
| recommended for detection of FBLIM1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 301-373 mapping at the C-terminus of FBLIM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 301-373 mapping at the C-terminus of FBLIM1 of human origin |
Application
Western blot analysis of FBLIM1 expression in WI 38 whole cell lysate.| Application Notes | recommended for detection of FBLIM1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 ELISA:1:1000~5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
