Application
Western blot analysis of HDAC1 expression in non-transfected: (A) and human HDAC1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HDAC1 expression in non-transfected: (A) and human HDAC1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HDAC1 expression in non-transfected 293T: (A), mouse HDAC1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.Application
Antibodies tested include LEF-1 (C-19): amd LEF-1 (N-17): (B), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (C), HDAC1 (H-11): , HDAC1 (C-19): and HDAC1 (H-11): (D), HDAC2 (C-8): , HDAC2 (H-54): and HDAC2 (C-19): (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.Application
Western blot analysis of HDAC1 expression in NIH/3T3 (A), HeLa (B), KNRK (C) and C32 (D) nuclear extracts.| Product Name | HDAC1 Antibody (H-51) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 432-482 of HDAC1 of human origin |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of HDAC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 432-482 of HDAC1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 432-482 of HDAC1 of human origin |
| Entrez Gene | 3065 |
|---|
Application
Western blot analysis of HDAC1 expression in non-transfected: (A) and human HDAC1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HDAC1 expression in non-transfected: (A) and human HDAC1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HDAC1 expression in non-transfected 293T: (A), mouse HDAC1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Antibodies tested include HDAC1 (H-15): , HDAC1 (C-19): and HDAC1 (H-11): (A), β-catenin (H-120): , β-catenin (C-18): and β-catenin (E-5): (B), E2F-4 (A-20): , E2F-4 (D-3): , E2F-4 (RK-13): and E2F-4 (C-20): (C), and p130 (C-20): , p130 (211.6): and p130 (H-125): (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA.
Application
Antibodies tested include LEF-1 (C-19): amd LEF-1 (N-17): (B), β-catenin (H-102): , β-catenin (C-18): and β-catenin (E-5): (C), HDAC1 (H-11): , HDAC1 (C-19): and HDAC1 (H-11): (D), HDAC2 (C-8): , HDAC2 (H-54): and HDAC2 (C-19): (E). Input control (A). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
Application
Western blot analysis of HDAC1 expression in NIH/3T3 (A), HeLa (B), KNRK (C) and C32 (D) nuclear extracts.| Application Notes | recommended for detection of HDAC1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
