Application
Western blot analysis of band 3 expression in HeLa (A), Caki-1 (B), HEL 92.1.7 (C), K-562 (D) and Hep G2 (E) whole cell lysates and human liver tissue extract (F).Application
Western blot analysis of band 3 expression in human fetal liver tissue extract.| Product Name | band 3 Antibody (L-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of band 3 of human origin by WB, IP and ELISA |
| Modification Notes | Band 3, also designated AE1, is an erythrocyte membrane glycoprotein that contributes to cell stuctural integrity and mediates exchange of chloride and bicarbonate across the phospholipid bilayer (1–3). The diverse functions of the approximately 900 amino acid protein are mediated by two distinct domains (2). The amino terminal domain, also known as cdb3 for cytoplasmic domain of erthrocyte membrane band 3, acts as an attachment site for the erythrocyte skeleton by binding ankyrin (2–4). The carboxy-terminal, membrane-associated domain carries out exchange transport of anions (2,5). Degradation of band 3 can generate an aging antigen known as senescent cell antigen, or SCA, which is expressed on old cells and marks them for removal by the immune system (1). An isoform of band 3, which lacks the first 65 amino acids and does not bind ankryin, is expressed in kidney (3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of band 3 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of band 3 of human origin by WB, IP and ELISA |
| Entrez Gene | 6521 |
|---|
Application
Western blot analysis of band 3 expression in HeLa (A), Caki-1 (B), HEL 92.1.7 (C), K-562 (D) and Hep G2 (E) whole cell lysates and human liver tissue extract (F).
Application
Western blot analysis of band 3 expression in human fetal liver tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
