Application
Western blot analysis of MEK-4 phosphorylation in untreated (A), UV irradiated (B) and lambda protein phosphatase treated and UV irradiated (C) HEK293 whole cell lysates.Application
Western blot analysis of MEK-4 phosphorylation in non-transfected 293T: (A), mouse MEK-4 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of phosphorylated MEK-4 expression in untreated (A) and UV-treated (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining.| Product Name | p-MEK-4 Antibody (Ser 80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 80 of MEK-4 of human origin |
| Modification | Phosphoralated |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38b (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 80 phosphorylated MEK-4 of human origin and correspondingly phosphorylated Ser 78 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 80 of MEK-4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 80 of MEK-4 of human origin |
| Entrez Gene | 6416 |
|---|
Application
Western blot analysis of MEK-4 phosphorylation in untreated (A), UV irradiated (B) and lambda protein phosphatase treated and UV irradiated (C) HEK293 whole cell lysates.
Application
Western blot analysis of MEK-4 phosphorylation in non-transfected 293T: (A), mouse MEK-4 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of phosphorylated MEK-4 expression in untreated (A) and UV-treated (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane staining.| Application Notes | recommended for detection of Ser 80 phosphorylated MEK-4 of human origin and correspondingly phosphorylated Ser 78 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
