Application
Western blot analysis of ICSBP expression in non-transfected: (A) and mouse ICSBP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ICSBP expression in NAMALWA whole cell lysate.| Product Name | ICSBP Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 357-426 mapping at the C-terminus of ICSBP of human origin |
| Modification Notes | ICSBP (Interferon (IFN) consensus sequence-binding protein, Interferon regulatory factor 8; IRF-8) is a transcription factor that is important for IFN-©-mediated signaling during dendritic cell and macrophage differentiation. ICSBP physically interacts with TRAF6 (between amino acid residues 356 and 305), and this interaction of ICSBP with TRAF6 modulates TLR signaling and may contribute to the cross-talk between IFN-© and TLR signal pathways. ICSBP antagonizes Bcr/Abl by downregulation of Bcl-2. ICSBP is known to interact with chromatin, and bind PU.1 in macrophages. ICSBP belongs to the IFN regulatory factor (IRF) family that also includes IRF-1, IRF-2, and ISGF-3. These proteins are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of IFN-å and IFN-∫. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ICSBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 357-426 mapping at the C-terminus of ICSBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 357-426 mapping at the C-terminus of ICSBP of human origin |
| Entrez Gene | 3394 |
|---|
Application
Western blot analysis of ICSBP expression in non-transfected: (A) and mouse ICSBP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ICSBP expression in NAMALWA whole cell lysate.| Application Notes | recommended for detection of ICSBP of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
