Application
Antibodies tested include p-hnRNP K (H-5): (A,B,C) and hnRNP K (H-300): (D,E,F).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of hnRNP K expression in NIH/3T3 (A), KNRK (B) and THP-1 (C) nuclear extracts.Application
Western blot analysis of hnRNP K expression in non-transfected: (A) and mouse hnRNP K transfected: (B) 293T whole cell lysates.Application
Western blot analysis of hnRNP K expression in HeLa (A), KNRK (B), Sol8 (C) and NIH/3T3 (D) nuclear extracts.| Product Name | hnRNP K Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of hnRNP K of human origin |
| Modification Notes | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of poly-peptides that contribute to mRNA transcription and pre-mRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins and their complexes are the major constituents of the spliceosome. The majority of hnRNP protein are localized to the nucleus, however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of hnRNP K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of hnRNP K of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of hnRNP K of human origin |
| Entrez Gene | 3190 |
|---|
Application
Antibodies tested include p-hnRNP K (H-5): (A,B,C) and hnRNP K (H-300): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of hnRNP K expression in NIH/3T3 (A), KNRK (B) and THP-1 (C) nuclear extracts.
Application
Western blot analysis of hnRNP K expression in non-transfected: (A) and mouse hnRNP K transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of hnRNP K expression in HeLa (A), KNRK (B), Sol8 (C) and NIH/3T3 (D) nuclear extracts.| Application Notes | recommended for detection of hnRNP K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
