Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.Application
Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.Application
Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic localization.| Product Name | NCoA-3 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1321-1400 of NCoA-3 of human origin |
| Modification Notes | Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. Several related transcriptional coactivators and corepressors have been described that work in concert with the steroid receptor family to either induce or repress transcription from hormone-responsive elements. This family includes GRIP1 (for GR interacting protein 1), also designated NCoA-2 or TIF-2; SRC-1 (for steroid receptor coactivator-1), also designated NCoA-1; and NCoA-3, also designated RAC3, ACTR, AIB-1 (for amplified in breast cancer) and p/CIP (for p300/CBP/co-integrator protein), which displays elevated expression in estrogen receptor positive ovarian and breast cancers and is required for the transcriptional activation of p300/CBP-dependent transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of NCoA-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1321-1400 of NCoA-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1321-1400 of NCoA-3 of human origin |
| Entrez Gene | 8202 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear staining of urothelial cells.
Application
Western blot analysis of NCoA-3 expression in K-562 whole cell lysate.
Application
Western blot analysis of NCoA-3 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear and cytoplasmic localization.| Application Notes | recommended for detection of NCoA-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
