Application
Western blot analysis of PRMT8 expression in non-transfected: (A) and human PRMT8 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of PRMT1 expression in non-transfected: (A) and mouse PRMT1 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of PRMT1 and PRMT8 expression in PC-3 nuclear extract.| Product Name | PRMT1 Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 166-300 of PRMT1 of human origin |
| Modification Notes | A class of proteins termed type 1 protein arginine N-methyltransferase (PRMTs) enzymes contribute to post-translational modification of RNA-binding proteins, but differ in substrate specificities, oligomerization properties, and subcellular localization. PRMT1, the predominant form in mammalian cells, is located in the nucleus. At the carboxy terminus, Interleukin enhancer-binding factor 3 (ILF3) binds PRMT1, thereby regulating PRMT1 activity. Alternative mRNA splicing of the PRMT gene results in three isoforms of PRMT1 that differ in their amino terminus regions, all of which are enzymatically active. PRMT8, also known as HRMT1L3 or HRMT1L4 (heterogenous nuclear ribonucleioprotein methyltransferase-like protein 4), is a distinct member of the type 1 PRMT family with tissue-specific expression and plasma membrane localization. PRMT8 is specifically expressed in the brain where it functions as an arginine methyltransferase with a possible role in neuronal differentiation. It is most closely related to PRMT1 and may have arisen through a gene duplication. PRMT8 can heterodimerize with PRMT1 and has similar substrate preference. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRMT1 and, to a lesser extent, PRMT8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 166-300 of PRMT1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 166-300 of PRMT1 of human origin |
| Entrez Gene | 3276 |
|---|
Application
Western blot analysis of PRMT8 expression in non-transfected: (A) and human PRMT8 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of PRMT1 expression in non-transfected: (A) and mouse PRMT1 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of PRMT1 and PRMT8 expression in PC-3 nuclear extract.| Application Notes | recommended for detection of PRMT1 and, to a lesser extent, PRMT8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
