Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Antibodies tested include p-RKIP (rSer 153): (A,B) and RKIP (FL-187): (C,D).Application
Western blot analysis of RKIP expression in 293T (A) and Hep G2 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of bile duct cells and hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of RKIP expression in PC-12 (A) whole cell lysate and rat brain (B) tissue extract.| Product Name | RKIP Antibody (FL-187) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-187 representing full length RKIP of human origin |
| Modification Notes | Raf kinase inhibitor protein (RKIP) is a cytosolic protein that was initially characterized as a phosphatidylethanolamine-binding protein (PBP) expressed in brain tissue and secreted from testis fluid. In addition, RKIP was identified by yeast two-hybrid screening of human T cell libraries directed at identifying proteins that associate with the BXB kinase domain of the serine/threonine kinase, Raf-1. Subsequent in vitro and in vivo studies indicate that RKIP binds to both the active and inactive forms of Raf-1 and thereby regulates the signaling cascade of the MAP kinase pathway. The specific association of RKIP with kinase-active Raf-1 competitively inhibits the binding and activation of the Raf-1 substrate MEK. RKIP, in turn, affects downstream MAP kinase signaling by decreasing the activation of MEK effector proteins, including ERK1 and ERK2, and the subsequent induction of AP-1 mediated transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RKIP and processed active peptide HCNP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also recommended for detection of Pebp-2 of mouse origin also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-187 representing full length RKIP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-187 representing full length RKIP of human origin |
| Entrez Gene | 5037 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Antibodies tested include p-RKIP (rSer 153): (A,B) and RKIP (FL-187): (C,D).
Application
Western blot analysis of RKIP expression in 293T (A) and Hep G2 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of bile duct cells and hepatocytes at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of RKIP expression in PC-12 (A) whole cell lysate and rat brain (B) tissue extract.| Application Notes | recommended for detection of RKIP and processed active peptide HCNP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also recommended for detection of Pebp-2 of mouse origin also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
