Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Tctex1 expression in Y79 (A), IMR-32 (B), T98G (C) and PC-3 (D) whole cell lysates.| Product Name | Tctex1 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-60 mapping at the N-terminus of Tctex1 of human origin |
| Modification Notes | Dyneins are multisubunit, high molecular weight ATPases that interact with microtubules to generate force by converting the chemical energy of ATP into the mechanical energy of movement. Cytoplasmic or axonemal Dynein heavy, intermediate, light and light-intermediate chains are all components of minus end-directed motors; the complex transports cellular cargos towards the central region of the cell. Axonemal Dynein motors contain one to three non-identical heavy chains and cause a sliding of microtubules in the axonemes of cilia and flagella in a mechanism necessary for cilia to beat and propel the cell. Cytoplasmic Dynein is an approximately 12 subunit complex of two heavy chains, two intermediate chains to anchor Dynein to its cargo, four smaller intermediate chains and several light chains. It performs functions necessary for cell survival such as organelle transport and centrosome assembly. The carboxy terminus of Dynein is important for microtubule-dependent motility and is highly conserved, while the amino terminal regions are more variable. Tctex1 is a cytoplasmic dynein light chain found in a complex with Na+ CP type Xå (SCN10A). Tctex1, also designated CW-1 or TCTEL1 is expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, prostate, testis, ovary, ileum and colon. Several proteins regulate Dynein activity, including dynactin, LIS1 and NudEL(NudE-like). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tctex1 of human origin, Dynlt1a, Dynlt1b, Dynlt1c and Dynlt1f of mouse origin, and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-60 mapping at the N-terminus of Tctex1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-60 mapping at the N-terminus of Tctex1 of human origin |
| Entrez Gene | 6993 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Tctex1 expression in Y79 (A), IMR-32 (B), T98G (C) and PC-3 (D) whole cell lysates.| Application Notes | recommended for detection of Tctex1 of human origin, Dynlt1a, Dynlt1b, Dynlt1c and Dynlt1f of mouse origin, and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
