Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-plasmic localization.Application
Western blot analysis of MEK-1 phosphorylation expression in untreated (A) and EGF treated (B) HeLa whole cell lysates.| Product Name | p-MEK-1 Antibody (Ser 222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 222 phosphorylated MEK-1 of m, r and h origin by WB, IP, IF and IHC(P); may cross-react with Ser 226 phosphorylated MEK-2 |
| Modification | Phosphoralated |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAFor MEKK1) positively regulates the kinase activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 222 phosphorylated MEK-1 of m r and h origin by WB IP IF and IHC(P) may cross-react with Ser 226 phosphorylated MEK-2 |
|---|---|
| Tissue Specificity | recommended for detection of Ser 222 phosphorylated MEK-1 of m, r and h origin by WB, IP, IF and IHC(P); may cross-react with Ser 226 phosphorylated MEK-2 |
| Entrez Gene | 5604 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-plasmic localization.
Application
Western blot analysis of MEK-1 phosphorylation expression in untreated (A) and EGF treated (B) HeLa whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
