Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing cytoplasmic staining of cells in endometrial stroma, myometrum and glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of TRPC3/6/7 expression in NRK whole cell lysate.| Product Name | TRPC3/6/7 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of TRPC3 of human origin |
| Modification Notes | Transient receptor potential cation (TRPC) channels are a superfamily of six transmembrane segment-spanning, gated cation channels. TRPC subtypes mediate store-operated Ca2+ entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. TRPC ion channels influence calcium-depletion induced calcium influx processes in response to chemo-, mechano- and osmoregulatory events. Human TRPC3 protein, also known as TRP3, is a cation channel that is predominantly expressed in brain. The activation of store-mediated Ca2+ entry in human cells likely occurs through the association between IP3R (inositol 1,4,5-trisphosphate receptors) and TRPC3. TRPC3 activity is also activated by DAG (diacylglycerol) independently of PKC (protein kinase C). Human TRPC6 is predominantly expressed in placenta, spleen, lung, small intestine and ovary. Also activated by diacylglycerol (DAG), TRPC6 comprises the a1-adrenoceptor-activated Ca(2+)-permeable cation channel. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TRPC3, TRPC6 and TRPC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of TRPC3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of TRPC3 of human origin |
| Entrez Gene | 7222 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing cytoplasmic staining of cells in endometrial stroma, myometrum and glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of TRPC3/6/7 expression in NRK whole cell lysate.| Application Notes | recommended for detection of TRPC3, TRPC6 and TRPC7 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
