Application
Western blot analysis of MKLP-1 expression in non-transfected: (A) and human MKLP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MKLP-1 expression in non-transfected 293T: (A), human MKLP-1 transfected 293T: (B) and K-562 (C) whole cell lyastes.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing nuclear and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of MKLP-1 expression in K-562 whole cell lysate.| Product Name | MKLP-1 Antibody (N-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of MKLP-1 of human origin |
| Modification Notes | The monoclonal antibody CHO1 detects a spindle antigen required for mitotic progression. Screening a HeLa cell cDNA expression library with this antibody has been shown to yield a cDNA predicted to encode a protein significantly related within its amino terminal half to the motor ends of members of the kinesin superfamily. Since this similarity does not extend further, it has been suggested that the CHO1 antigen, now designated MKLP-1 (mitotic kinesin-like protein-1), represents a novel kinesin. Sequence analysis has also been shown to predict that MKLP-1 possesses features typical of nuclear proteins. Immunocytological studies have demonstrated that MKLP-1 moves from the nucleus early in mitosis and then to the midbody after cytokinesis. MKLP-1 has been shown to bundle antiparallel microtubules in vitro and to move them at rates comparable to spindle elongation in vivo. A hamster homolog of MKLP-1, designated CHO1 antigen, has also been isolated. Although apparently functionally equivalent with respect to microtubule bundling activity, there are significant differences between the human and hamster proteins at their C-termini, possibly due to alternative splicing or the presence of more than one MKLP-1 locus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of MKLP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of MKLP-1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of MKLP-1 of human origin |
| Entrez Gene | 9493 |
|---|
Application
Western blot analysis of MKLP-1 expression in non-transfected: (A) and human MKLP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MKLP-1 expression in non-transfected 293T: (A), human MKLP-1 transfected 293T: (B) and K-562 (C) whole cell lyastes.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing nuclear and cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of MKLP-1 expression in K-562 whole cell lysate.| Application Notes | recommended for detection of MKLP-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
