Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of JNK p54 expression in COS (A) and JNK2-transfected COS (B) whole cell lysates.Application
Antibodies tested include JNK1 (F-3): (A-D), JNK1 (C-17)-G: (E-H) and JNK2 (FL): (I-L).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear and cytoplasmic staining of epidermal cells.| Product Name | JNK Antibody (FL) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | produced by immunization with full length (amino acids 1-424) human JNK produced in E. coli |
| Modification Notes | c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of JNK2, JNK1 p46 and JNK3 of mouse, rat, human, zebrafish, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | produced by immunization with full length (amino acids 1-424) human JNK produced in E. coli |
|---|---|
| Tissue Specificity | produced by immunization with full length (amino acids 1-424) human JNK produced in E. coli |
| Entrez Gene | 5601 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.
Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of JNK2 expression in non-transfected: (A) and human JNK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of JNK p54 expression in COS (A) and JNK2-transfected COS (B) whole cell lysates.
Application
Antibodies tested include JNK1 (F-3): (A-D), JNK1 (C-17)-G: (E-H) and JNK2 (FL): (I-L).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear and cytoplasmic staining of epidermal cells.| Application Notes | recommended for detection of JNK2, JNK1 p46 and JNK3 of mouse, rat, human, zebrafish, Drosophila and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
