Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of 14-3-3 β expression in A-431 (A), K-562 (B), U-937 (C) and NIH/3T3 (D) whole cell lysates.| Product Name | 14-3-3 β Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of 14-3-3 β of human origin |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 14-3-3 β and, to a lesser extent, other 14-3-3 family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of 14-3-3 &beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of 14-3-3 β of human origin |
| Entrez Gene | 7529 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of 14-3-3 β expression in A-431 (A), K-562 (B), U-937 (C) and NIH/3T3 (D) whole cell lysates.| Application Notes | recommended for detection of 14-3-3 β and, to a lesser extent, other 14-3-3 family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
