Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondrial localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.Application
Western blot analysis of ACSL4 expression in non-transfected: (A) and human ACSL4 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of ACSL4 expression in Hep G2 whole cell lysate.| Product Name | ACSL4 Antibody (H-53) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 623-675 mapping near the C-terminus of ACSL4 of human origin |
| Modification Notes | Acyl-CoA synthetases, also known as long-chain fatty-acid CoA synthases (FACL) or palmitoyl-CoA ligases, include ACSL1-6, which are all single-pass membrane proteins localizing to the mitochondrion, microsome or peroxisome. FACL proteins are important for synthesis of cellular lipids and for ∫-oxidation degradation. Specifically, ACSL proteins catalyze the activation of long-chain fatty acids to acyl-CoAs, which can be metabolized to form CO2, triacylglycerol (TAG), phospholipids (PL) and cholesteryl esters (CE). ACSL3 preferentially utilizes laurate, myristate, arachidonate and eicosapentaenoate among saturated and unsaturated long chain fatty acids. FACL3 is expressed as two isoforms in various tissues, including brain, heart, placenta, prostate, skeletal muscle, testis and thymus. FACL4 preferentially utilizes arachidonate and is abundant in steroidogenic tissues. FACL4 may modulate female fertility and uterine prostaglandin production. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of short isoform and long isoform of ACSL4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 623-675 mapping near the C-terminus of ACSL4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 623-675 mapping near the C-terminus of ACSL4 of human origin |
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing mitochondrial localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.
Application
Western blot analysis of ACSL4 expression in non-transfected: (A) and human ACSL4 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of ACSL4 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of short isoform and long isoform of ACSL4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
