Application
Western blot analysis of RUNX2 expression in MDCK whole cell lysate.Application
Western blot analysis of RUNX2 expression in adenovirus RUNX2 infected MDCK cells. Kindly provided by Midory Thorikay and Maarten van Dinther, Department of Cellular Biochemistry, The Netherlands Cancer Institute Amsterdam.| Product Name | RUNX2 Antibody (M-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 294-363 mapping to an internal region of RUNX2 of mouse origin |
| Modification Notes | The mammalian Runt-related transcription factor (RUNX) family comprises three members, RUNX1 (also designated AML-1, PEBP2åB, CBFA2), RUNX2 (also designated AML-3, PEBP2åA, CBFA1, Osf2) and RUNX3 (also designated AML-2, PEBPåC, CBFA3). RUNX family members are DNA-binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. RUNX2 is essential for skeletal mineralization in that it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones. Regulating RUNX2 expression may be a useful therapeutic tool for promoting bone formation. Mutations in the C-terminus of RUNX2 are associated with cleidocranial dysplasia syndrome, an autosomal-dominant skeletal dysplasia syndrome that is characterized by widely patent calvarial sutures, clavicular hypoplasia, supernumerary teeth, and short stature. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, equine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 294-363 mapping to an internal region of RUNX2 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 294-363 mapping to an internal region of RUNX2 of mouse origin |
| Entrez Gene | 860 |
|---|
Application
Western blot analysis of RUNX2 expression in MDCK whole cell lysate.
Application
Western blot analysis of RUNX2 expression in adenovirus RUNX2 infected MDCK cells. Kindly provided by Midory Thorikay and Maarten van Dinther, Department of Cellular Biochemistry, The Netherlands Cancer Institute Amsterdam.| Application Notes | recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, equine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
