Application
Western blot analysis of BRUNOL4 expression in WI 38 whole cell lysate.| Product Name | BRUNOL4 Antibody (S-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of BRUNOL4 of human origin |
| Modification Notes | Members of the CELF (CUG-BP1- and ETR-3-like factor) family are RNA-binding proteins implicated in the regulation of pre-mRNA alternative splicing. The CELF family includes 6 members. CELF4, also designated BRUNOL4, mediates exon inclusion and/or exclusion in pre-mRNAs that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically, BRUNOL4 activates exon 5 inclusion of cardiac isoforms of Troponin T-C during heart remodeling at the juvenile to adult transition, and promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. BRUNOL4 contains 3 RRM (RNA recognition motif) domains and binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of Troponin T-C pre-mRNA. BRUNOL4 is strongly expressed in cerebellum, hippocampus, amygdala, temporal and frontal cortex and frontal lobes. Disruption of the gene encoding BRUNOL4 results in idiopathic epilepsy, a common human disorder that leads to severe seizures. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of BRUNOL4 of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with isoforms BRUNOL4-2, BRUNOL4-3 or BRUNOL4-5 also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of BRUNOL4 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of BRUNOL4 of human origin |
| Entrez Gene | 56853 |
|---|
Application
Western blot analysis of BRUNOL4 expression in WI 38 whole cell lysate.| Application Notes | recommended for detection of BRUNOL4 of mouse and human origin by WB, IP, IF and ELISA non cross-reactive with isoforms BRUNOL4-2, BRUNOL4-3 or BRUNOL4-5 also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
