Application
Western blot analysis of Ribosomal Protein S19 expression in K-562 whole cell lysate (A) and HeLa nuclear extract (B).Application
Western blot analysis of Ribosomal Protein S19 expression in K-562 (A) and HeLa (B) whole cell lysates.| Product Name | Ribosomal Protein S19 Antibody (FL-145) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-145 representing full length Ribosomal Protein S19 of human origin |
| Modification Notes | Ribosomal subunits are synthesized in the nucleus and mature 40S and 60S subunits are exported stoichiometrically into the cytoplasm. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. Ribosomal proteins have the ability to pass through the nu-clear envelope in the native state, making them the largest of the structures accommodated by the nuclear pore complexes. The nuclear export of ribosomal subunits is a unidirectional, saturable and energy-dependent process. Ribosomal Protein S19 (RPS19) is a 145 amino acids protein expressed in various human adult tissues, including bone marrow, peripheral blood, spleen, liver and nonhematopoietic tissues. RPS19 expression decreases during terminal erythroid differentiation; a deficiency of RPS19 blocks proliferation of immature erythroid progenitor cells altogether. Muta-tions in the RPS19 gene are linked with Diamond-Blackfan anemia (DBA), a congenital, hypo-plastic, red cell aplasia that occasionally presents with physical anomalies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ribosomal Protein S19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-145 representing full length Ribosomal Protein S19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-145 representing full length Ribosomal Protein S19 of human origin |
| Entrez Gene | 6223 |
|---|
Application
Western blot analysis of Ribosomal Protein S19 expression in K-562 whole cell lysate (A) and HeLa nuclear extract (B).
Application
Western blot analysis of Ribosomal Protein S19 expression in K-562 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Ribosomal Protein S19 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
