Application
Western blot analysis of IL-27Rα expression in rat thymus (A), mouse eye (B), mouse skin (C) and rat liver (D) tissue extracts.| Product Name | IL-27Rα Antibody (M-191) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 25-215 mapping near the N-terminus of IL-27Rα of mouse origin |
| Modification Notes | IL-27 is a heterodimeric cytokine that consists of EBI3, an IL-12p40-related protein, and p28, a IL-12p35-related polypeptide. IL-27 triggers expansion of antigen-specific naive CD4-positive T cells and promotes polarization towards a Th1 phenotype with expression of gamma-interferon. IL-27 contributes to the development of an adaptive immune response through its action on CD4-positive T cells, and also directly acts on cells of the innate immune system. IL-27 protein levels increase upon activation of antigen-presenting cells. IL-27 protein induces orphan cytokine receptor IL-27R (WSX-1)-dependent clonal expansion of naive but not memory CD4(+) T cells. IL-27 signaling through IL-27R and gp130 also induces phosphorylation of Stat1-5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-27Rα of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 25-215 mapping near the N-terminus of IL-27R&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 25-215 mapping near the N-terminus of IL-27Rα of mouse origin |
Application
Western blot analysis of IL-27Rα expression in rat thymus (A), mouse eye (B), mouse skin (C) and rat liver (D) tissue extracts.| Application Notes | recommended for detection of IL-27Rα of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
