Application
Western blot analysis of Selenoprotein K expression in Jurkat whole cell lysate.| Product Name | Selenoprotein K Antibody (H-48) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 37-84 mapping within an internal region of Selenoprotein K of human origin |
| Modification Notes | Selenoprotein K (SELK), also known as HSPC030, is a 94 amino acid that belongs to the selenoprotein family which contains the rare twenty-first amino acid, selenocysteine (sec) that is used in ribosome-mediated protein synthesis. The 3' UTR of selenoprotein genes have a common stem-loop structure known as the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a sec codon rather than as a stop signal. Unlike the other 20 amino acids in protein, sec is biosynthesized from its tRNA. Widely expressed, Selenoprotein K localizes to endoplasmic reticulum and is found at high levels in heart, where it may function as an antioxidant. Overexpression of Selenoprotein K attenuates intracellular reactive oxygen species level and guards cardiomyocytes from oxidative stress-induced toxicity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Selenoprotein K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 37-84 mapping within an internal region of Selenoprotein K of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 37-84 mapping within an internal region of Selenoprotein K of human origin |
| Entrez Gene | 58515 |
|---|
Application
Western blot analysis of Selenoprotein K expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of Selenoprotein K of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
