Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Bcl10 expression in non-transfected 293T: (A), mouse Bcl10 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Bcl10 expression in non-transfected 293T: (A), human Bcl10 transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Bcl10 expression in non-transfected control (A) and Bcl10 siRNA transfected (B) WEHI-231 cells. Blot probed with Bcl10 (H-197): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of Bcl10 expression in CTLL-2 (A), MM-142 (B), BYDP (C), RAW 264.7 (D) and WEHI-231 (E) whole cell lysates.Application
Western blot analysis of Bcl10 expression in K-562 whole cell lysate.| Product Name | Bcl10 Antibody (H-197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-197 mapping at the N-terminus of Bcl10 of human origin |
| Modification Notes | Bcl10, also designated CIPER, c-CARMEN and mE10, was first identified as a gene truncated or mutated in MALT B cell lymphomas and other tumor types. Bcl10 is homologous to the equine herpesvirus-2 E10 gene and, like E10, it contains an N-terminal caspase recruitment domain (CARD). Expression of Bcl10 has been shown to induce NF˚B activation in a NIK-dependent pathway, and research indicates that the CARD domain is essential for this activation; although in a separate study, Bcl10 by itself did not induce JNK or NF˚B activation. Overexpression of Bcl10 has been shown to induce apoptosis in a manner dependent on CARD-mediated oligomerization. Bcl10 has also been shown to play a role in processing of caspase-9 to its active dimer. Other studies have shown that Bcl10 is not mutated in many human tumors and lymphomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Bcl10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-197 mapping at the N-terminus of Bcl10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-197 mapping at the N-terminus of Bcl10 of human origin |
| Entrez Gene | 8915 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Bcl10 expression in non-transfected 293T: (A), mouse Bcl10 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Bcl10 expression in non-transfected 293T: (A), human Bcl10 transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Bcl10 expression in non-transfected control (A) and Bcl10 siRNA transfected (B) WEHI-231 cells. Blot probed with Bcl10 (H-197): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of Bcl10 expression in CTLL-2 (A), MM-142 (B), BYDP (C), RAW 264.7 (D) and WEHI-231 (E) whole cell lysates.
Application
Western blot analysis of Bcl10 expression in K-562 whole cell lysate.| Application Notes | recommended for detection of Bcl10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
