Application
Western blot analysis of TAO2 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Product Name | p-TAO2 Antibody (Ser 181) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 181 phosphorylated TAO2 of mouse, rat, human, Xenopus and zebrafish origin by WB and IP; also reactive with additional species, including canine |
| Modification | Phosphoralated |
| Modification Notes | Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase Ste20. Ste20 is involved in relaying signals from G protein-coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MEK kinase. Thousand and one amino acid protein 2 (TAO2), also designated prostate-derived Ste20-like kinase 1 (PSK1) or kinase from chicken homolog C (KFC-C), belongs to the Ste20 subfamily of the Ser/Thr protein kinase family. TAO2 acts as an activator of the JNK MAP kinase pathway through the specific activation of MKK3 and MKK6 kinases. It is a multi-pass membrane protein detected in cytoplasmic vesicle membranes. TAO2 is ubiquitously expressed with highest levels found in brain and testis. Mouse, rat and human TAO2 are phosphorylated on Ser 181 in the activation loop, which allows the conformational activation of TAO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 181 phosphorylated TAO2 of mouse rat human Xenopus and zebrafish origin by WB and IP also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Ser 181 phosphorylated TAO2 of mouse, rat, human, Xenopus and zebrafish origin by WB and IP; also reactive with additional species, including canine |
| Entrez Gene | 9344 |
|---|
Application
Western blot analysis of TAO2 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
