Application
Antibodies tested include p-c-Abl/Arg (Tyr 393/439): (A,B) and c-Abl (K-12): (C,D).Application
Immunopero-xidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Blots were probed with p-c-Abl/Arg (Tyr 393/439): pre-incubated with cognate phosphorylated peptide (A,B).| Product Name | p-c-Abl/Arg Antibody (Tyr 393/439) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 393 phosphorylated c-Abl and correspondingly phophorylated Tyr 439 Arg of m and h origin by WB, IP, IF and IHC(P) |
| Modification | Phosphoralated |
| Modification Notes | The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including chronic myelocytic leukemia (CML), in which it undergoes a (9;22) chromosomal translocation and produces the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein tyrosine kinase. The related protein tyrosine kinase Arg, also designated Abl2, contains an SH2 and an SH3 domain. Arg has been shown to interact with and to phosphorylate c-Crk. Both c-Abl and Arg undergo post-translational phosphorylation on multiple amino acid residues. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 393 phosphorylated c-Abl and correspondingly phophorylated Tyr 439 Arg of m and h origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 393 phosphorylated c-Abl and correspondingly phophorylated Tyr 439 Arg of m and h origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 25 |
|---|
Application
Antibodies tested include p-c-Abl/Arg (Tyr 393/439): (A,B) and c-Abl (K-12): (C,D).
Application
Immunopero-xidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Blots were probed with p-c-Abl/Arg (Tyr 393/439): pre-incubated with cognate phosphorylated peptide (A,B).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
