Application
Western blot analysis of NFκB p65 phosphorylation in non-transfected: (A) and mouse NFκB p65 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of phosphorylated NFκB p65 expression in untreated RAW 264.7 (A) IL-1-treated RAW 264.7 (B), untreated HeLa (C) and TNFα-treated HeLa (D) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-NFκB p65 Antibody (Ser 536) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 536 of NFκB p65 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NF˚B (p50 and p65) and the Drosophila maternal morphogen, dorsal. Both proteins specifically bind to DNA sequences that are the same or slight variations of the 10 bp ˚B sequence in the immunoglobulin k light chain enhancer. This same sequence is also present in a number of other cellular and viral enhancers. The DNA binding activity of NFkB is activated and NFkB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity.The NFkB transcription factor is a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit, also referred to as Rel A, is functionally related to c-Rel p75 and RelB p68. NF˚Bp65 is phosphorylated at Serine-311 as a response to protein kinase C zeta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 536 phosphorylated NFκB p65 of human origin correspondingly phosphorylated Ser 534 of mouse origin and correspondingly phosphorylated Ser 535 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 536 of NF&kappa B p65 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 536 of NFκB p65 of human origin |
| Entrez Gene | 5970 |
|---|
Application
Western blot analysis of NFκB p65 phosphorylation in non-transfected: (A) and mouse NFκB p65 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of phosphorylated NFκB p65 expression in untreated RAW 264.7 (A) IL-1-treated RAW 264.7 (B), untreated HeLa (C) and TNFα-treated HeLa (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 536 phosphorylated NFκB p65 of human origin correspondingly phosphorylated Ser 534 of mouse origin and correspondingly phosphorylated Ser 535 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
