Application
Western blot analysis of SSH3 expression in A-431 whole cell lysate.| Product Name | SSH3 Antibody (H-229) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 431-659 mapping at the C-terminus of SSH3 of human origin |
| Modification Notes | SSH3 (Slingshot homolog 3), also known as SSH3L, is a 659 amino acid protein that localizes to both the nucleus and cytoplasm and is a human homolog of the Drosophila slingshot (ssh) protein. Functioning as a protein phosphatase, SSH3 is thought to regulate actin filament dynamics through its control of proteins such as ADF (actin-depolymerizing factor) and Cofilin. The ADF/Cofilin family consists of stimulus-responsive mediators that rapidly depolymerize and disassemble F-actin in a stoichiometric manner and can be deactivated by a variety of kinases. SSH3 acts to catalytically dephosphorylate the ADF/Cofilin proteins, thereby reactivating them and allowing them to resume their control over actin dynamics. SSH3 contains one tyrosine-protein phosphatase domain and is expressed as five isoforms due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SSH3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 431-659 mapping at the C-terminus of SSH3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 431-659 mapping at the C-terminus of SSH3 of human origin |
| Entrez Gene | 54961 |
|---|
Application
Western blot analysis of SSH3 expression in A-431 whole cell lysate.| Application Notes | recommended for detection of SSH3 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
