Application
Western blot analysis of IκB-α phosphorylation in untreated (A), TNFα treated (B) and TNFα and lambda protein phosphatase treated (C) HEK293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Product Name | p-IκB-α Antibody (Ser 32/36) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 32/36 of IκB-α of human origin |
| Modification | Phosphoralated |
| Modification Notes | On the basis of both functional and structural considerations, members of the I˚B family of proteins can be divided into four groups. The first of these groups, I˚B-å, includes the avian protein pp40 and the mammalian MAD-3, both of which inhibit binding of p50–p65 NFkB complex or Rel protein to their cognate binding sites but do not inhibit the binding of p50 homodimer to ˚B sites, suggesting that the I˚B-å family binds to the p65 subunit of p50–p65 heterocomplex through ankyrin repeats. The second member of the I˚B family is represented by a protein designated I˚B-∫. The third group of I˚B proteins is represented by I˚B-©, a protein identical in sequence with the C-terminal domain of the p110 precursor of NFkB p50 and expressed predominantly in lymphoid cells. An additional I˚B family member has been identified as I˚B-e, a protein which has several phosphorylated forms and is primarily found complexed with Rel A and/or c-Rel. There is a consensus phosphorylation site for CKII between residues 269-299, and within this range there are three phosphorylation sites that important for constitutive phosphorylation and intrinsic stability of I˚B-å: Ser 283, Thr 291 and Thr 299. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 32 and Ser 36 dually phosphorylated IκB-α of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 32/36 of I&kappa B-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 32/36 of IκB-α of human origin |
| Entrez Gene | 4792 |
|---|
Application
Western blot analysis of IκB-α phosphorylation in untreated (A), TNFα treated (B) and TNFα and lambda protein phosphatase treated (C) HEK293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Ser 32 and Ser 36 dually phosphorylated IκB-α of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
