Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ETEA expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.Application
Western blot analysis of ETEA expression in non-transfected 293T: (A), human ETEA transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of ETEA expression in Jurkat whole cell lysate.| Product Name | ETEA Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 147-434 mapping at the C-terminus of ETEA of human origin |
| Modification Notes | ETEA, also designated UBX domain-containing protein 8 (UBXD8), is a 445-amino acid protein associated with atopic dermatitis (AD), a chronic noncontagious relapsing inflammatory skin disease characterized by eczematous skin lesions and also referred to as eczematous dermatitis. Other atopic diseases such as hay fever, asthma and conjunctivitis often occur along with AD. ETEA shows higher expression in T cells and eosinophils of patients with AD than in T cells and eosinophils of unaffected individuals. T cells are influential in the regulation of the inflammatory process of this disease. The persistence of AD is attributed to dysregulated apoptosis in T cells, eosinophils, and keratinocytes. ETEA may be involved in the resistance to apoptosis in T cells and eosinophils of AD patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ETEA of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 147-434 mapping at the C-terminus of ETEA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 147-434 mapping at the C-terminus of ETEA of human origin |
| Entrez Gene | 23197 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ETEA expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.
Application
Western blot analysis of ETEA expression in non-transfected 293T: (A), human ETEA transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of ETEA expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of ETEA of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
