Application
Western blot analysis of CNOT4 expression in Jurkat whole cell lysate.Application
Western blot analysis of CNOT4 expression in non-transfected (A) and human CNOT4 transfected (B) 293 whole cell lysates.| Product Name | CNOT4 Antibody (O-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCR4 NOT transcription complex subunit 4,CCR4-associated factor 4 ,CLONE243,CNOT 4,Cnot4 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT4 (CCR4-NOT transcription complex subunit 4), also known as CCR4-associated factor 4 and E3 ubiquitin-protein ligase CNOT4, is a 575 amino acid protein that is a subunit of the CCR4-NOT complex. CNOT4 contains one C3H1-type zinc finger, one RING-type zinc finger and one RRM (RNA recognition motif) domain. Via its RING domain, CNOT4 binds E2 ubiquitin ligases. CNOT4 functions as a UbcH5B-dependent ubiquitin-protein ligase (E3 ligase). There are eight isoforms of CNOT4 that are expressed as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB:1:100~1:200 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | CCR4 NOT transcription complex subunit 4 CCR4-associated factor 4 CLONE243 CNOT 4 Cnot4 |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Entrez Gene | 4850 |
|---|
Application
Western blot analysis of CNOT4 expression in Jurkat whole cell lysate.
Application
Western blot analysis of CNOT4 expression in non-transfected (A) and human CNOT4 transfected (B) 293 whole cell lysates.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
