Application
Western blot analysis of MIPEP expression in non-transfected (A) and human MIPEP transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | MIPEP Antibody (J-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MIPEP of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | MIPEP (Mitochondrial intermediate peptidase) is a 713 amino acid protein that performs the final step in processing nuclear-encoded proteins that are targeted the mitochondrial inner membrane or matrix. MIPEP is primarily involved in the maturation of oxidative phosphorylation-related proteins and is accordingly expressed at highest levels in tissues that have high oxygen consumption, such as skeletal muscle, brain, heart and pancreas. Significantly, human MIPEP is the functional homolog of yeast YMIP, a protein that promotes mitochondrial iron uptake and is regulated by the yeast homolog of frataxin, which is required for mitochondrial iron efflux. Due to the likely regulatory effect that human frataxin has on it, MIPEP may be implicated in the clinical manifestations of Friedreich ataxia, an autosomal recessive neurodegenerative disease caused by deficiency of frataxin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MIPEP of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MIPEP of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4285 |
|---|
Application
Western blot analysis of MIPEP expression in non-transfected (A) and human MIPEP transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
