Application
Western blot analysis of NUDT9 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing cytoplasmic localization.| Product Name | NUDT9 Antibody (V-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NUDT9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | NUDT9 (nudix (nucleoside diphosphate linked moiety X)-type motif 9), also known as NUDT10, is a 350 amino acid protein belonging to the superfamily of nudix hydrolases. Expressed as two isoforms that are produced as a result of alternative splicing events, NUDT9 isoform 1 localizes to the mitochondria and is the predominant isoform. NUDT9 is known to function as a highly specific adenosine diphosphate ribose pyrophosphatase that hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5'-phosphate. It has been suggested that NUDT9 may be involved in the regulation of the menstrual cycle and may be related to the proliferation of glandular cells in the human endometrium. NUDT9 consist of two distinct domains: a proteolytically resistant C-terminal domain that retains essentially full specific ADPR pyrophosphatase activity, and a proteolytically labile N-terminal portion that functions to enhance the affinity of the C-terminal domain for ADPR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NUDT9 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of NUDT9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 53343 |
|---|
Application
Western blot analysis of NUDT9 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
