Application
Western blot analysis of Atg4b expression in Jurkat whole cell lysate..Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.Application
Western blot analysis of Atg4b expression in 293T whole cell lysate.| Product Name | Atg4b Antibody (K-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Atg4b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagy-related gene (Atg) proteins. Atg4b (ATG4 autophagy related 4 homolog B), also known as APG4B, AUTL1 or KIAA0943, is a 393 amino acid protein that localizes to the cytoplasm and and belongs to the peptidase C54 family. Expressed in heart, pancreas, brain, liver and skeletal muscle, Atg4b functions as a cysteine protease that cleaves the C-terminal part of target proteins, such as GABARAP and MAP1LC3, and plays an essential role in autophagy. Atg4b exists as multiple alternatively spliced isoforms and is functionally inhibited by N-ethylmaleimide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Atg4b of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Atg4b of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 23192 |
|---|
Application
Western blot analysis of Atg4b expression in Jurkat whole cell lysate..
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.
Application
Western blot analysis of Atg4b expression in 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
