Application
Western blot analysis of TRB-1 expression in non-transfected: (A) and mouse TRB-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TRB-1 expression in T-47D whole cell lysate.| Product Name | TRB-1 Antibody (D-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRB-1 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. TRB-1 (tribbles homolog 1), also known as C8FW, GIG2 or TRB1, is a 372 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase superfamily. Expressed ubiquitously with highest expression in bone marrow, thyroid gland, skeletal muscle and pancreas, TRB-1 interacts with MAPK kinases and is thought to regulate the activation of MAP kinses, possibly controlling MAP kinase cascades. The gene encoding TRB-1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRB-1 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRB-1 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 10221 |
|---|
Application
Western blot analysis of TRB-1 expression in non-transfected: (A) and mouse TRB-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TRB-1 expression in T-47D whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
