Application
Western blot analysis of HDAC11 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded testicle tumor tissue showing nuclear localization.| Product Name | HDAC11 Antibody (Y-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HDAC11 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Histone deacetylases (HDACs) play an important role in the modification of chromatin structure and thus in the suppression and activation of transcription and cellular differentiation. There are 11 members in the HDAC family that are classified into four classes. Class I HDACs represent homologs of the yeast histone deacetylase RPD3, class II HDACs share strong homology with the yeast histone deacetylase HDA1, class III HDAC are closely related to the yeast SIR2 protein, and class IV HDACs comprises Histone deacetylase 11 (HDAC11)-related enzymes. HDAC11 contains 347 amino acid residues. HDAC11 contains conserved residues in the catalytic core regions shared by both class I and II mammalian HDAC enzymes. Expression of HDAC11 is high in the kidney, heart, brain, skeletal muscle, and testis, and it localizes to the cell nucleus. The human gene encoding for HDAC11 maps to chromosome 3p25. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HDAC11 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HDAC11 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 79885 |
|---|
Application
Western blot analysis of HDAC11 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded testicle tumor tissue showing nuclear localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
