Application
Western blot analysis of GPBP expression in HeLa whole cell lysate.| Product Name | GPBP Antibody (S-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GPBP of human origin by WB, IP and ELISA |
| Modification Notes | GPBP, also known as COL4A3BP (collagen, type IV, alpha 3 (Goodpasture antigen) binding protein), CERT or STARD11, is a 624 amino acid protein that localizes to the cytoplasm, as well as to the Golgi apparatus and the endoplasmic reticulum, and contains one PH domain and one START domain. Expressed in a variety of tissues, GPBP interacts with COL4A3 and is thought to mediate the intracellular trafficking of ceramides (lipid molecules composed of a sphingosine and a fatty acid), possibly regulating lipid function throughout the cell. In addition, GPBP acts as a kinase that is able to phosphorylate the N-terminal region of COL4A3, an event which may play a role in the development of Goodpasture disease, a rare syndrome that can lead to severe kidney and lung damage. Two isoforms of GPBP exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GPBP of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GPBP of human origin by WB, IP and ELISA |
| Entrez Gene | 10087 |
|---|
Application
Western blot analysis of GPBP expression in HeLa whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
