Application
Western blot analysis of HIRIP3 expression in Jurkat (A), HL-60 (B) and K-562 (C) nuclear extracts.Application
Western blot analysis of HIRIP3 expression in Jurkat nuclear extract.| Product Name | HIRIP3 Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-100 mapping at the N-terminus of HIRIP3 of human origin |
| Modification Notes | The HIRIP3 (HIRA interacting protein 3) locus encodes for a 556 amino acid protein that directly interacts with the HIRA histone chaperone. It also interacts weakly with core histones, Histone H2B and Histone H3. HIRIP3 is a heavily phosphorylated nuclear protein and it is found throughout the cell cycle. It is phosphorylated by casein kinase II. HIRIP3 may play a role in chromatin function and histone metabolism. A region (approximately 60 amino acids in length) at the C-terminus of HIRIP3 is highly conserved among vertebrates and it contains residues that are invariantly charged, polar, and hydrophobic. Two isoforms of HIRIP3 exists due to alternative splicing. Isoform 1 is predominately expressed in skeletal muscles and isoform 2 is expressed in the liver and the heart. Human HIRA homologs are thought to be responsible for the DiGeorge syndrome and related developmental disorders. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HIRIP3 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-100 mapping at the N-terminus of HIRIP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-100 mapping at the N-terminus of HIRIP3 of human origin |
| Entrez Gene | 8479 |
|---|
Application
Western blot analysis of HIRIP3 expression in Jurkat (A), HL-60 (B) and K-562 (C) nuclear extracts.
Application
Western blot analysis of HIRIP3 expression in Jurkat nuclear extract.| Application Notes | recommended for detection of HIRIP3 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
