Application
Western blot analysis of SAP 114 expression in 721B whole cell lysate.Application
Western blot analysis of SAP 114 expression in HeLa (A) and K-562 (B) whole cell lysates.| Product Name | SAP 114 Antibody (Z-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SAP 114 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | SAP 114 (spliceosome associated protein 114), also known as PRP21, PRPF21, SF3A120 or SF3A1, is a 793 amino acid ubiquitously expressed nuclear protein belonging to the SURP protein family and contains two SURP motif repeats and one ubiquitin-like domain. SAP 114 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the first subunit of the complex, SAP 114 interacts with subunit 2 (SAP 62) and subunit 3 (SAP 61) via its SURP motifs. SAP 114 is also thought to be involved in the assembly of the E complex, a critical regulator of cell cycle progression from G1 into S phase in mammalian cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SAP 114 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SAP 114 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 10291 |
|---|
Application
Western blot analysis of SAP 114 expression in 721B whole cell lysate.
Application
Western blot analysis of SAP 114 expression in HeLa (A) and K-562 (B) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
