Application
Western blot analysis of UBE2J1 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing cytoplasmic and membrane localization.| Product Name | UBE2J1 Antibody (L-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of UBE2J1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Ubiquitination is an important molecular mechanism by which abnormal or short-lived proteins are targeted for degradation by the concerted efforts of at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2J1 (Ubiquitin-conjugating enzyme E2 J1), also known as Ubc6p, CGI-76, NCUBE1, HSPC153 or HSPC205, is a 318 amino acid single-pass type IV membrane protein that belongs to the E2 ubiquitin-conjugating enzyme family and is involved in protein degradation. Localized to the membrane of the endoplasmic reticulum (ER), UBE2J1 catalyzes the attachment of ubiquitin to misfolded membrane proteins, thereby targeting them for proteasomal destruction. This ATP-dependent reaction yields AMP, a diphosphate and a ubiquitin-tagged protein and may be a method of quality control within the ER. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of UBE2J1 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of UBE2J1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 51465 |
|---|
Application
Western blot analysis of UBE2J1 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic and membrane localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
