Application
Western blot analysis of PYGM expression in non-transfected (A) and human PYGM transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization.| Product Name | PYGM Antibody (U-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PYGM of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose. Phospho-fructose kinase-2/fructose 2,6-bisphosphatase (PFK-2) stimulates the synthesis and degradation of fructose 2,6-bisphosphate. Glycogen phosphorylase (also known as GP) is an allosteric enzyme important in carbohydrate metabolism. Its activity is regulated through either noncovalent binding of metabolites or by covalent modification. Glycogen phosphorylase catalyzes the phosphorylation of glycogen to Glc-1-P. There are three genes which encode the brain, liver and muscle forms of glycogen phosphorylase, PYGB, PYGL and PYGM. Because of its fundamental role in the metabolism of glycogen, glycogen phosphorylase has been a target for the design of inhibitory compounds, which could be valuable in the therapeutic treatment of type 2 diabetes mellitus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PYGM of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PYGM of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5837 |
|---|
Application
Western blot analysis of PYGM expression in non-transfected (A) and human PYGM transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
